Experimental Evidence for the Fröhlich Hypothesis

Gunther Schmidl
gschmidl@gmx.at

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Abstract

Over the years, numerous experiments have been conducted with millimeter wave radiation; some of the data can be used to try and prove or disprove Fröhlich's Hypothesis. This paper gives an overview of experiments from both the Former Soviet Union (over the last decade) and more recent developments in the field and discusses the results.

Contents

1  Introduction
    1.1  Why Millimeter Waves?
    1.2  Organisation of this Paper
2  Physicochemical Effects
3  Influence at the Subcellular, Cellular and Tissue Levels
    3.1  Effects on Human Erythrocytes
    3.2  Effects on Growth Rate
    3.3  Effects on Proteins, Chromosomes and Genes
    3.4  Effects on Membranes
    3.5  Other effects
4  Influence at the Organism Level
    4.1  Effects on Receptors
    4.2  Animal Studies
        4.2.1  Influence on Surface Wounds
        4.2.2  Influence on Organism Self-Repair
        4.2.3  Influence on Tumor Growth
        4.2.4  Other Findings
    4.3  Clinical Studies
        4.3.1  General Information
        4.3.2  Problems
        4.3.3  Examples
        4.3.4  Side Effects
5  Conclusion

1  Introduction

1.1  Why Millimeter Waves?

Radiation of cells with microwaves, and especially millimeter waves1, is known to cause the temperature in those cells to rise and thus enable or influence biological processes such as growth.

It is, however, believed that millimeter waves cause a second kind of biological effects to emerge, unrelated to those caused by increased temperature (ie. at very low radiation intensity). The absence of coherent oscillations in the millimeter wave range in the natural environment - the absence of noise - would make that frequency band convenient for inter- and intra-cell communication.

H. Fröhlich formulated a hypothesis stating that energy in cells was not thermalized but instead stored in molecular vibration modes. According to his theory, coherent dipole vibrations generate an electromagnetic field that is used for long-range interactions between cells using water as a medium. This effect should be visible at normal temperatures and occur in all living things.

Specifically, Fröhlich's Hypothesis states that cells are able to recognize each other at a distance and be attracted or repelled. A second important part of the hypothesis is the vibrational model: trigger action of microwaves causing the excitation of coherent electric vibrations pumped by energy derived from metabolism. [Frö81]

Radiation of many biological effects with millimeter waves in the frequency region of 5 ×1010 Hz show sharp frequency resonances and have nonthermal effects on many biological activities. [Frö75]

Many speculations have been made referring to the Fröhlich model, and a large number of experiments has been carried out.

1.2  Organisation of this Paper

Starting from the ``lowest level'', physicochemical effects, and working its way up to the organism level, this paper strives to summarize experimental evidence for the existence or non-existence of effects according to Fröhlich's Hypothesis gathered over the last decade.

It relies on data from the Former Soviet Union, where research on millimeter waves has been pursued extensively, and current developments in the United States and England.

Since much of the data is taken from [PYP+98], it is reproduced here as short summaries or in tabular format to avoid unnecessary duplication.

2  Physicochemical Effects

During the course of experimentation, specific effects of millimeter waves on water or biomolecular solutions have been found. They include:

Further studies showed that the specific absorption rate (SAR) of flat structures with high water content is, apart from radiation frequency, dependent on the following factors:

Fröhlich's model [Frö81] states that MMW radiation supplements the amount of biological pumping of energy, thus triggering biological effects which would have occurred at a later stage without the influence of radiation. Nonthermal action of microwaves at 2450 MHz seems to be established without a doubt.

3  Influence at the Subcellular, Cellular and Tissue Levels

[Note: there have been many experiments with millimeter wave radiation that are irrelevant to the topic of this paper; therefore, they are not included here. This includes, for example, suppression of bioluminescence.]

3.1  Effects on Human Erythrocytes

Rowlands et al. [RSL+81] noticed a small attraction between erythrocytes in plasma before their actual contact. The attraction was no longer detectable when the membrane potential was decreased to near zero, the ATP reserves were exhausted or cellular organization was deranged.

The paper cites this as evidence for Fröhlich's Hypothesis, especially since the effect is completely reversible (although it modifies structure and results in ion shifts). The membrane potential dropped from 15 to 7.1 mV after metabolic depletion, probably below a threshold for the Fröhlich effect2.

The interaction causes cells even beyond the distances of Brownian motion to move towards each other and form into linear aggregates (roleaux). In experiments [RSE82], fewer than 30 cells remained unassociated after 15 minutes.

An assumption of the Smoluchowski theory is that all the particles are the same size; mixtures of erythrocytes from different species will aggregate when suspended in isotonic solutions of certain macromolecules - however, cells of the same size (and thus the same species) show a preference to adhere to each other. Experimentally, mixtures of dispersed embryonic heart and liver cells sort themselves out. [RSE82]

Embryonic stem cells are an important field in modern biological research. A major breakthrough would be the ability to use millimeter wave stimulation to cause stem cells to evolve into different organs, perhaps using data gathered from the above-mentioned experiments.

3.2  Effects on Growth Rate

Numerous experiments have been carried out concerning the influence on growth rate at the cellular level. In several cases, however, the experimental results could not be reproduced in later attempts, either due to lack of documentation or because of unknown but significant differences in the experiment set-up.

What follows is a list of the findings of MMW influence on growth rate; results that could not be reproduced are found at the end of the list and are marked with an asterisk.

organism duration wave power effects
S. cerivisiae 50 min 46 GHz 0.03 mW/cm2 Fluctuation in growth rate
Barley seeds 20 min 61.5 GHz 0.1 mW/cm2 36% increase in synchronized cell division
Spirulina platensis 30 min 7.1 mm 2.2 mW/cm2 growth rate doubled, photosynthetic action increased 1.5-fold

3.3  Effects on Proteins, Chromosomes and Genes

Experiments [Frö75] on DNA in weak electric fields have led to the conclusion that DNA does not have a permanent dipole moment; experiments in stronger fields, however, led to the conclusion of a permanent dipole. This supports the claim of highly polar metastable states made by Fröhlich's Hypothesis: excitation of the polar state would lead to breaking of electrical symmetry and have far-reaching biological effects.

According to Fröhlich, expected interaction frequencies at the protein level are between 1012 and 1014 Hz, while those for RNA and DNA are around 109 Hz. [Frö81]

Measurements of proteins and amino acids showed exact cancellation of the intrinsic dipole moments of the individual amino acids in, for example, myoglobin.

Fröhlich suggests [Frö75] that protein conformation places charged groups outside to interact with water to account for the aforementioned cancellation. He mentions that one would expect minimal interaction energy, which has to go beyond nearest neighbour interaction - in fact, it requires interaction of charged groups. This could be significant for the self-organization of larger units, for example the attraction of a protein to a specific site; the pairing of homologous chromosomes during meiosis; the travelling of newly synthesized proteins to their destinations in the cell. [Frö81]

It has been conclusively proven that exposure to MMW does not cause mutagenic or recombinant effects in genes and chromosomes; there is evidence, however, that it might increase the probability of recombinant effects caused by ultraviolet radiation:

Thermal effects have not been ruled out as the cause of these changes.

A disputed experiment using the Anomalous Viscosity Time Dependence technique (AVTD) showed that sharp resonance in chromosomal structures could be caused by energies as low as 10-19 W/cm2, peaking between 10-17 and 10-8 W/cm2. Interpretation of this data is, however, uncertain, since AVTD is not normally used in cell biology.

3.4  Effects on Membranes

Biological membranes are usually around 10-6 cm thick and carry a potential difference of the order of 100 mV. The field is used in nerve conduction, but membranes of non-nerve cells also carry a potential difference. A section of the membrane oscillating represents an electric dipole; proteins built into the membrane may also oscillate at lower or higher frequencies.

Experimental evidence for this is summarized below, taken from [Frö78]:

Together with chromosomes, membranes are thought to be the major target for MMW radiation. A large number of studies and experiments is focused on membrane effects; the results are summarized in the table below.

[Note: some experiments used intermittent exposure; these will be marked with (IE/nn), where nn is the number of minutes between exposures. All times are given in minutes, all frequencies in GHz and all powers in mW/cm2 unless otherwise noted.]

organism duration frequency power effects
SR, rat skeletal muscle 5 61 4 Ca++ uptake increases 23%. (IE/15)
 20   Ca++ uptake increases 27%.
 40   Ca++ uptake reaches 48%.
Ca++-activated K+ channels in kidney cells 20-30 42.25 0.1 Increases activity of channels with low initial activity and inhibits channels with high initial activity4.
Giant alga cells 30-60 41, 50, 71 5 Chloride current drops to 0; no recovery for 10-14 h.
  49, 70, 76  Chloride curent increases 200-400%; reversible within 30-40 min.
Frog sciatic nerve 50-110 77.7 10 CAP5 falls 10-fold; sensitization to frequency.
 ? 41.14-41.54 0.02-2.6 frequency-dependent CAP decrease (bell curve).
Striatic muscle and cardiac pacemaker cells 1.5 54-78 0.1-0.15 Deceleration of transmembrane potential loss; reduction of overshoot voltage, action potential amplitued and conduction velocity.

Effects of MMW radiation in membranes is not monotonic; peaks of effects are not fixed at certain frequencies and change with the complexity of the organism involved.

3.5  Other effects

Other effects at low radiation, described by Chernyakov et al., include synchronization of neuron ``firing'', enhanced respiration, reduction of contractility in cardiac cells and altered membrane calcium binding.

One of the speculations referring to Fröhlich's Hypothesis is the existence of cooperative oscillatory dipole states in a particular stage of their life. [RBP81] showed experimentally that cells in their reproductive state attract highly polarizable particles more than poorly polarizable particles, decreasing with the increase of the conductivity.

4  Influence at the Organism Level

As millimeter waves do not penetrate the skin deeply, reactions at the organism level are believed to take place at the skin or shallow subcutaneous regions. They are then somehow propagated, but it is currently not understood how. Indeed, there is no obvious connection between cellular-level and organism-level reactions; the subject remains open for speculation.

4.1  Effects on Receptors

One of the propagation theories believes peripheral receptors and their nerves to be responsible for the propagation of MMW effects. The following table summarizes the experiments carried out on peripheral receptors.

[Note: All times are given in minutes, all frequencies in GHz and all powers in mW/cm2 unless otherwise noted.]

area duration frequency power effects
electroreceptor Lorencini ? 33-55 1-4 increased firing rate
   10+ inhibition of firing
rat upper thoracic vertebrae 20 55, 73 10 arrhythmia, no changes in body temperature
 150 51, 61, 73  sudden death
frog bladder 20 42.04-42.34 10 alterations of spontaneous afferent firing in the bladder nerve
rat skin mechanoreceptors, hind limb sole 35 55 0.75 changes in threshold and latency in response to stimuli
  61 2.90  
  73 7.81  

In addition, heart rate changes in frogs could be induced by irradiating remote skin areas, even if the heart was entirely denervated.

In experiments with humans, 30-80% of test subjects could detect exposure of the hand to MMW radiation (37.7, 42.25, 53.57 GHz at 5-15 mW/cm2); higher frequencies caused more subjects to detect radiation than higher power.

Fröhlich mentions [Frö81] that electric fields at 10-20 Hz, as well as in the microwave region, can influence the EEG severely; during certain periods, large regions of the brain oscillate coherently.

4.2  Animal Studies

Suprisingly few animal studies were carried out by researchers in the former Soviet Union; what little material there is mostly concerns itself with the influence of MMW radiation on surface wound healing processes.

4.2.1  Influence on Surface Wounds

4.2.2  Influence on Organism Self-Repair

4.2.3  Influence on Tumor Growth

Fröhlich believes [Frö78] that a cell which has lost its correct vibrational frequency can no longer contribute to a collective vibration and might disrupt the cell division control barrier, causing tumor growth if more and more cells go out of synchronization. This is highly speculative at best, and no evidence exists.

4.2.4  Other Findings

Several other experiments were conducted, and the findings are summarized below.

4.3  Clinical Studies

It is interesting to note that while few studies of effects on animals exist, the Russian government has officially approved MMW therapy, and more than 3,000,000 people were treated in hospitals and specialized centers between 1977 and 1995 (the date of the report by Lebedeva and Betskii). There are hundreds of publications on the clinical use of MMW radiation and a commercial market for MMW generators.

4.3.1  General Information

Depending on the patient's illness, the location, wavelength and duration of irradiation are selected by the physician. ``Standard'' values for most diseases vary between 15 to 60 min/day for 8-15 days.

Three models are used frequently:

The effects of MMW radiation are disputed: while many authors claim that MMW monotherapy was able to help even patients with incurable diseases and hardly ever showed any side effects, others claimed that the success depended very much on the patient's condition and sensitivity to the radiation. Yet another group of studies claims that MMW was inferior to conventional therapy every time, and there are even studies claiming MMW radiation did not have any effects whatsoever.

The following list of conditions reportedly successfully treated with MMW radiation must therefore, in light of the above, be taken with a grain of salt (and it may indeed seem that nothing was left off the list):

4.3.2  Problems

About 30% of studies report the use of MMW therapy on either known acupuncture points or areas to which the patient proves sensitive. Therapeutic effects could be explained by thermal effects, as an intensity of 10 mW/cm2 may be enough to cause acupuncture- or acupressure-like effects through heating.

No rationale for the use of the above-mentioned frequencies and intensities or the application to certain skin areas could be found; unfortunately, many studies do not conform to traditional quality criteria and must be used with caution.

4.3.3  Examples

4.3.4  Side Effects

MMW radiation is generally well tolerated by patients, though some studies claimed side effects in up to 26% of patients. Local low-power MMW irradiation is believed to cause adversary health effects and requires special attention.

Reported side effects in treatment of cardiovascular diseases in 200 patients, while facilitating recovery in 56-77% of cases (depending on pathology), included the following:

# of cases side effect
9 elevated blood pressure
7 diencephalic crisis / paroxysm
6 enhanced menstrual bleeding
5 fever
3 angina attacks

Reported side effects in treatment of stage II essential hypertension in 124 patients included the following:

# of cases side effect
33 blood pressure fluctuations, headaches
16 reactions of the autonomous nervous system6
6 sleepiness, nightmares, weakness, irritability
2 hypertensive crisis

In therapy of 528 patients with various diseases, three patients developed hives after 5-7 days of treatment; it spread over the whole body, but disappeared 2-10 days after the treatment ended. The reason is unknown.

5  Conclusion

In many of the studies summarized in the previous chapters, effects markedly different from those caused by heating have been found; in others, the power of MMW radiation was too low to cause thermal effects.

The problem with publications from the former Soviet Union is their reliability, since (as previously mentioned) many are flawed in one way or another, most of the time missing control mechanisms such as sham-exposed control groups, a double-blind component in which neither researcher nor patient know whether the treatment is a placebo or exact specifications of the conditions in which the experiment was performed.

The reasons why millimeter wave radiation works are unknown; Fröhlich's Hypothesis tries to explain. Unfortunately though, the effects of MMW can not be conclusively proven to be caused by long-range communication of cells, even if the hints are strong - irradiation of skin causing changes in internal organs, for example.

Fröhlich's Hypothesis will have to remain a hypothesis for the time being.

Bibliography

References

[Frö75]
H. Fröhlich. The extraordinary dielectric properties of biological materials and the action of enzymes. Proceedings of the Natural Academic Sciences of the USA, 72:4211-5, 1975.

[Frö78]
H. Fröhlich. Coherent electric vibrations in biological systems and the cancer problem. IEEE Transactions on Microwave Theory and Techniques, MTT-26(8):613-7, 1978.

[Frö81]
H. Fröhlich. The biological effects of microwaves and related questions. Advances in Electronics and Electron Physics, 53:85-152, 1981.

[JvTP96]
Frantisek Jelínek, Jaroslav Saroch, Viktor Trkal, and Jirí Pokorný. Measurement system for experimental verification of fröhlich electromagnetic field. Journal of Bioelectrochemistry and Bioenergetics, 41:35-8, 1996.

[PYP+98]
Andrei G. Pakhomov, Aykel Yahya, Olga N. Pakhomova, Bruce E. Stuck, and Michael E. Murphy. Current state and implications of research on biological effects of millimeter waves: A review of the literature. Journal of Bioelectromagnetics, 19:393-413, 1998.

[RBP81]
S. C. Roy, Tim Braden, and H. A. Pohl. Possibility of existence of pseudoferroelectric state in cells: some experimental evidence. Physics Letters, 83A(3):142-4, 1981.

[RS82]
S. Rowlands and L. S. Sewchand. A quantum mechanical interaction of human erythrocytes. Canadian Journal of Physiology and Pharmacology, 60:52-9, 1982.

[RSE82]
S. Rowlands, L. S. Sewchand, and E. G. Enns. Further evidence for a fröhlich interaction of erythrocytes. Physics Letters, 87A(5):256-60, 1982.

[RSL+81]
S. Rowlands, L. S. Sewchand, R. E. Lovlin, J. S. Beck, and E. G. Enns. A fröhlich interaction of human erythrocytes. Physics Letters, 82A(8):436-8, 1981.

[VRL+93]
Marten H. Vos, Fabrice Rappaport, Jean-Christophe Lambry, Jacques Breton, and Jean-Louis Martin. Visualization of coherent nuclear motion in a membrane protein by femtosecond spectroscopy. Nature, 363:320-5, 1993.


Footnotes:

1Electromagnetic oscillations in the range between 30 and 300 GHz

2This is unproven.

3However, it is not known whether they are caused by intermediate chemical products

4These effects could be reproduced by exposing the membrane to irradiated bathing solution (30 minutes at 42.25 GHz / 2 mW/cm2). There was no change in pH or Ca++ concentration of the solution. The mechanisms of this are not yet understood, but highly promising.

5compound action potential

6whole-body shivering, sweating, heart pains together with skin paling or reddening


File translated from TEX by TTH, version 2.89. Minor corrections by the author.